Simultaneous determination of pregnanediol and pregnanolone in urinary extracts by gas chromatography.

HAVE ltEl’ORTED a comparison of methods for determining urniary pregnanediol and have shown that enzymatic hydrolysis of preglianelhiOl conjugate, preliminary purification using silica gel chromatography, anti gas chromatographic quantitation was the prefered method (1). hi this report we show a simple modification of this procedure that enables the simultaneous determination of pregnanediol ($-pregnaiie-3, 20diol) and pregnailolone (5$-pregnane-3, 20-one).